In vitro synthesis of polyhydroxyalkanoates using thermostable acetyl-CoA synthetase, CoA transferase, and PHA synthase from thermotorelant bacteria

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• 作者：Kenji Tajima¹ ; ^{ktajima@eng.hokudai.ac.jp} ; Xuerong Han¹ ; ^§ ; ; Yoshiki Hashimoto¹ ; Yasuharu Satoh¹ ; Toshifumi Satoh¹ ; Seiichi Taguchi¹ ; ²
• 关键词：In vitro synthesis ; Polyhydroxyalkanoate ; Thermophilic bacteria ; Pelotomaculum thermopropionicum ; Thermus thermophilus
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：122
• 期：6
• 页码：660-665
• 全文大小：795 K
• 卷排序：122

文摘

A gene of acetyl-CoA synthetase (ACSPt) was cloned from Pelotomaculum thermopropionicum JCM10971. A gene of CoA transferase (CTTt) was cloned from Thermus thermophilus JCM10941. ACSPt and CTTt were confirmed to have high thermostability. In vitro PHA synthesis was successfully performed by ACSPt, CTTt, PhaC1SG(STQK). Yields of PHAs at 37°C were 1.4-fold higher than those of the system with non-thermostable enzymes.