UHPLC/Q-TOFMS-based metabolomics for the characterization of cold and hot properties of Chinese materia medica

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• 作者：Yang Wang^a ; Shujun Zhou^a ; Meng Wang^a ; Shuying Liu^b ; Yuanjia Hu^a ; Chengwei He^a ; Peng Li^a ; Jian-Bo Wan^a ; ^{jbwan@umac.mo" class="auth_mail" title="E-mail the corresponding author}
• 关键词：C ; cold ; CMM ; Chinese material medica ; ESI ; electrospray ionization ; H ; hot ; LCAT ; lecithin-cholesterol acyltransferase ; LPC ; lysophosphatidylcholine ; LPE ; lysophosphatidylethano-lamine ; PGI2 ; prostacyclin ; PLA2 ; phospholipase ; PLS-DA ; partial least squares discriminant analysis ; QC ; quality control ; ROS ; reactive oxygen species ; RSD ; relative standard deviation ; TCM ; traditional Chinese medicine ; UHPLC/Q-TOFMS ; ultra-high performance liquid chromatography- quadrupole time-of-flight mass spectro
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：17 February 2016
• 年：2016
• 卷：179
• 期：Complete
• 页码：234-242
• 全文大小：1574 K

文摘

The cold/hot property of Chinese materia medica (CMM) and the application of its corresponding knowledge in the diagnosis, differentiation and treatment of diseases have been considered to be the extremely important part of traditional Chinese medicine (TCM). As highly abstracted TCM theory, the cold/hot property of CMMs is still not fully understood and remains to be elucidated by systems biology approach. The cold and hot properties of CMM are mainly defined by the response of the body to a given CMM. Metabolomics is a promising systems biology method to profile entire endogenous metabolites and monitor their fluctuations related to an exogenous stimulus. Thus, a metabolomics approach was applied to characterize the cold and hot properties of CMMs.

Material and methods

Mice were intragastrically administered three selected cold property CMMs (i.e., Rheum palmatum L., radix et rhizoma; Coptis chinensis Franch, rhizome and Scutellaria baicalensis Georgi, radix) and three hot property CMMs (i.e., Cinnamomum cassia (L.) J. Presl, cortex; Zingiber officinale Roscoe, rhizoma and Evodia rutaecarpa (Juss.) Benth., fructus) once daily for one week. The comprehensive metabolome changes in the plasma of mice after treatment with cold or hot property CMMs were characterized by ultra-high performance liquid chromatography/time of flight mass spectrometry (UHPLC/Q-TOF-MS), and the potential biomarkers related to cold and hot properties of CMM were explored.

Results

Metabolites perturbation in plasma occurs after treatment with cold CMMs and hot CMMs in mice, and 15 and 16 differential biomarkers were identified to be associated with the cold and hot properties of CMMs, respectively. Among them, LPC (18:0), LPC (18:1), LPC (20:4) and LPC (20:5) showed decreased trends in the cold property CMM treated groups, but increased in the hot property CMM treated groups.

Conclusions

There is a strong connection between the cold/hot property of CMMs and lysophosphatidylcholines metabolism. This study offers new insight into CMM properties and their clinical application.