Molecular cloning of the murine cMOAT ATPase
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- 作者：Fritz ; Friederike ; Chen ; Jing ; Hayes ; Paula ; Sirotnak ; F.M.
- 关键词：Canalicular multispecific anion transporter ; Murine cDNA ; ORF ; open reading frame ; UTR ; untranslated region ; cMOAT ; canalicular multispecific anion transporter
- 刊名：Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression
- 出版年：2000
- 出版时间：July 24, 2000
- 年：2000
- 卷：1492
- 期：2-3
- 页码：531-536
- 全文大小：1.44 M
文摘
cMOAT encodes an ATPase within the family of cMOAT/MRP ATPases that functions as an ATP dependent, multispecific anion transporter within the canalicular surface of hepatocytes that has pharmacologic significance. We describe here the cloning of a murine cMOAT cDNA isolated from mouse liver. The open reading frame of this cDNA incorporates 4627 nucleotides encoding 1309 amino acids with 77.5 % and 86.7 % identity with the human and rat encoded amino acids, respectively. Northern blotting showed that the expression of cMOAT mRNA occurs primarily in mouse liver in the form of two variants with approximately 5.6 and 7.8 kb of sequence each. cMOAT mRNA was also detected in mouse kidney at a low level but was not detected in other mouse organs or tumors except the Hep 1-6 murine hepatoma where expression was also in the form of the same two mRNA variants.