RNA decay machines: Deadenylation by the Ccr4-Not and Pan2-Pan3 complexes

详细信息    查看全文

• 作者：Elmar Wahle^a ; ^{ewahle@biochemtech.uni-halle.de} ; G. Sebastiaan Winkler^b ; ^{sebastiaan.winkler@nottingham.ac.uk}
• 关键词：mRNA decay ; Ribonuclease ; CNOT6 ; CNOT6L ; CNOT7 ; CNOT8
• 刊名：Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
• 出版年：2013
• 出版时间：June-July, 2013
• 年：2013
• 卷：1829
• 期：6-7
• 页码：561-570
• 全文大小：1038 K

文摘

Shortening and removal of the 3¡ä poly(A) tail of mature mRNA by poly(A)-specific 3¡ä exonucleases (deadenylases) is the initial and often rate-limiting step in mRNA degradation. The majority of cytoplasmic deadenylase activity is associated with the Ccr4-Not and Pan2-Pan3 complexes. Two distinct catalytic subunits, Caf1/Pop2 and Ccr4, are associated with the Ccr4-Not complex, whereas the Pan2 enzymatic subunit forms a stable complex with Pan3. In this review, we discuss the composition and activity of these two deadenylases. In addition, we comment on generic and specific mechanisms of recruitment of Ccr4-Not and Pan2-Pan3 to mRNAs. Finally, we discuss specialised and redundant functions of the deadenylases and review the importance of Ccr4-Not subunits in the regulation of physiological processes. This article is part of a Special Issue entitled: RNA Decay mechanisms.