Dog shedding oocysts of Neospora caninum: PCR diagnosis and molecular phylogenetic approach
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- 作者：&Scaron ; lapeta ; Jan R. ; Modrý ; David ; Kyselová ; Iveta ; Horej&scaron ; Radek ; et. al.
- 关键词：Neospora ; Hammondia ; Toxoplasma ; Coccidia ; Species-specific PCR ; Identification ; Diagnosis
- 刊名：Veterinary Parasitology
- 出版年：2002
- 出版时间：November 11, 2002
- 年：2002
- 卷：109
- 期：3-4
- 页码：157-167
- 全文大小：184 K
文摘
Results of molecular determination of a dog isolate of Neospora caninum in the Czech Republic are presented. Colorless bisporocystic oocysts measuring 10–13μm×10–11μm were recovered from feces and used for DNA isolation. A diagnostic PCR procedure using previously described molecular methods was performed to determine the species. The N. caninum species-specific primers based on the Nc5 region produced a positive result, while primers specific for Hammondia heydorni rDNA internal transcribed spacer 1 (ITS1) was negative. Sequencing and phylogenetic comparison of ITS1 rDNA and the D2 domain of the large subunit rDNA (D2 LSU) determined our isolate to be N. caninum. Phylogenetic analysis of closely related genera Toxoplasma, Neospora and Hammondia based on ITS1 and D2 LSU robustly distinguished three clades: (i) Toxoplasma gondii + Hammondia hammondi, (ii) N. caninum + Neospora hughesi, and (iii) H. heydorni. Based on phylogenetic relationships we propose three acceptable suggestions to solve the problem of taxonomy of these genera.