Neutrophil extracellular traps in the intestinal mucosa of Eimeria-infected animals

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• 作者：Tamara Muñ ; oz-Caro^# ; Liliana Machado Ribeiro da Silva^# ; Zaí ; da Renterí ; a-Solis ; Anja Taubert ; Carlos Hermosilla ; ^{Carlos.R.Hermosilla@vetmed.uni-giessen.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Eimeria bovis ; Eimeria arloingi ; Neutrophil extracellular traps ; Apicomplexa ; Coccidiosis
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：6
• 期：4
• 页码：301-307
• 全文大小：1741 K

文摘

To investigate the presence of neutrophil extracellular traps (NETs) in vivo by analysing intestinal sections from experimentally Eimeria bovis- and naturally Eimeria arloingi-infected animals.

Methods

Intestinal samples of Eimeria arloingi- and Eimeria bovis-infected animals were analysed by using immunohistochemical and fluorescence approach by using monoclonal antibodies.

Results

Classical NET components were confirmed by co-localization of extracellular DNA being decorated with neutrophil elastase and histones in Eimeria-infected tissue samples. Here, extrusion of NETs was exclusively detected in intestinal polymorphonuclear neutrophils infiltrating Eimeria-infected sites. In vivo NETs were either found in close proximity or in direct contact to different Eimeria stages suggesting a stage-independent process. NETs were also found within the gut lumen driven by polymorphonuclear neutrophils that were contacting released oocysts.

Conclusions

We postulate that NETs might play an important role in innate defence reactions in coccidiosis therefore significantly altering the outcome of infection.