Calibration-free concentration analysis for an analyte prone to self-association

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• 作者：Hiroshi Imamura ; Shinya Honda ; ^{s.honda@aist.go.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Surface plasmon resonance ; Calibration-free concentration analysis ; Self-association ; Aggregation
• 刊名：Analytical Biochemistry
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：516
• 期：Complete
• 页码：61-64
• 全文大小：204 K

文摘

Calibration-free concentration analysis (CFCA) based on surface plasmon resonance uses the diffusion coefficient of an analyte to determine the concentration of that analyte in a bulk solution. In general, CFCA is avoided when investigating analytes prone to self-association, as the heterogeneous diffusion coefficient results in a loss of precision. The derivation for self-association of the analyte was presented here. By using the diffusion coefficient for the monomeric state, CFCA provides the lowest possible concentration even though the analyte is self-associated.