Crystal structure of nanoKAZ: The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine
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- 作者：Yuri Tomabechia ; Takamitsu Hosoyab ; Haruhiko Eharaa ; Shun-ichi Sekinea ; Mikako Shirouzua ; Satoshi Inouyec ; sinouye@jnc-corp.co.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：β-barrel structure ; Fatty acid-binding protein ; Catalytic site ; Dinoflagellate luciferase ; Coelenterazine analogs
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：29 January 2016
- 年：2016
- 卷：470
- 期：1
- 页码：88-93
- 全文大小：1564 K
文摘
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The 19 kDa protein (KAZ) of Oplophorus luciferase is a catalytic component.
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The crystal structure of a mutant KAZ (nanoKAZ) was determined.
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The structure consists of 11 β-strands and 4 α-helices, forming a β-barrel.
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The structure of nanoKAZ is similar to those of fatty acid-binding proteins.
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The coelenterazine-binding site might be in a central cavity of the protein.