Differential patterns of expression of neuropeptide Y throughout abstinence in outbred Swiss mice classified as susceptible or resistant to ethanol-induced locomotor sensitization
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- 作者：Ricardo Font茫o de Pauli ; C谩ssia Canha Coelhoso ; Carolina Tesone-Coelho ; Aless ; ra Linardi ; Luiz Eug锚nio Mello ; Dartiu Xavier Silveira ; Jair Guilherme Santos-Junior
- 关键词：Neuropeptide Y ; Locomotor sensitization ; Ethanol abstinence ; Behavioral variability ; Mice
- 刊名：Alcohol
- 出版年：February, 2014
- 年：2014
- 卷：48
- 期：1
- 页码：63-72
- 全文大小：1104 K
文摘
Several studies have focused on the negative emotional state associated with drug abstinence. The peptide NPY plays an important role given its involvement in drug addiction, anxiety, and mood disorders. Interestingly, it is well established that outbred Swiss mice exhibit a prominent behavioral variability to ethanol-induced locomotor sensitization. Here, we investigated whether mice that were either susceptible or resistant to ethanol sensitization differed in their NPY expression during abstinence. The mice were treated daily with ethanol (2聽g/kg, i.p.) or saline for 21 days. According to the locomotor activity after the last injection, the ethanol group was classified as sensitized (EtOH_High) or non-sensitized (EtOH_Low). To evaluate NPY expression, some of the mice were sacrificed at 18聽h or 5 days of abstinence, and others were challenged at the 5th day of abstinence with ethanol (1.4聽g/kg) and sacrificed after 1.5聽h. At 5 days of abstinence, NPY expression increased in the orbital cortex, dorsomedial striatum, and dentate gyrus in the EtOH_High mice. These changes were counteracted by the ethanol challenge. In the EtOH_Low mice, NPY expression increased in the dentate gyrus only after 18聽h of abstinence. Lastly, a decreased level of NPY was found in the prelimbic cortex of the EtOH_Low mice at 5 days of abstinence, and this was reversed by ethanol challenge. Therefore, behavioral variability in ethanol sensitization confers differential neurochemical features during the subsequent abstinence, including distinct patterns of NPY expression.