A method for obtaining RNA from Hemileia vastatrix appressoria produced in planta, suitable for transcriptomic analyses
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- 作者：Andreia Loureiroa ; b ; Helena Gil Azinheiraa ; b ; hgazinheira@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Maria do Cé ; u Silvaa ; b ; Pedro Talhinhasa ; b
- 关键词：Coffee leaf rust ; Nitrocellulose film ; Pre-penetration fungal structures ; RNA integrity ; Transcriptomics
- 刊名：Fungal Biology
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：119
- 期：11
- 页码：1093-1099
- 全文大小：1004 K
文摘
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Hemileia vastatrix can differentiate appressoria in vitro in oil:collodion membranes.
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In vitro appressoria differentiate at similar rates as in planta appressoria.
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Nail polish replicas can remove intact appressoria from coffee leaf surface.
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Polish-collected appressoria RNA is of good quality and suitable for cDNA synthesis.
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cDNA from polish-collected appressoria can be used for transcriptomic analyses.