Molecular cloning, characterization, and expression analysis of a heat shock protein (HSP) 70 gene from Paphia undulata
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- 作者：Xiangwei Wua ; b ; Jing Tana ; Mingyi Caia ; Xiande Liua ; xdliu@jmu.edu.cn" class="auth_mail
- 关键词：cDNA ; DNA complementary to RNA ; HSP70 ; heat shock protein 70 gene ; PCR ; polymerase chain reaction ; RT-PCR ; reverse transcriptase PCR ; qRT-PCR ; quantitative RT-PCR ; RACE ; rapid amplification of cDNA ends ; UTR ; untranslated region ; ORF ; open reading frame ; kDa ; kilodalton ; Hsps ; Heat shock proteins ; Hsp70 ; heat shock protein ; Hsc70 ; heat shock cognate protein 70 ; PuHSC70 ; heat shock cognate protein 70 from Paphia undulata ; PuHSC70 ; heat shock cognate protein 70 gene of Paphia undulata ; mRNA ; messa
- 刊名：Gene
- 出版年：15 June 2014
- 年：2014
- 卷：543
- 期：2
- 页码：275-285
- 全文大小：2253 K
文摘
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A full-length heat shock protein 70 cDNA in Paphia undulata was cloned.
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The HSP70 mRNA is ubiquitously expressed in P. undulata.
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The HSP70 mRNA initially increased and then decreased under heat shock stress.
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The HSP70 mRNA decreased under high salinity stress in the gill and digestive gland.