Valuation of brewer's spent grain using a fully recyclable integrated process for extraction of proteins and arabinoxylans

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• 作者：Elsa Vieira ; M. Ang茅lica M. Rocha ; Elisabete Coelho ; Ol铆via Pinho ; Jorge A. Saraiva ; Isabel M.P.L.V.O. Ferreira ; Manuel A. Coimbra
• 关键词：Brewer's spent grain ; Proteins ; Arabinoxylans ; Recycling
• 刊名：Industrial Crops and Products
• 出版年：January, 2014
• 年：2014
• 卷：52
• 期：Complete
• 页码：136-143
• 全文大小：917 K

文摘

The objective of this work was to design an integrated process to valuate brewer's spent grain (BSG) proteins and arabinoxylans (AX) to be used as food ingredients. For this purpose, a sequential extraction of proteins and AX from BSG with increasing alkali (KOH or NaOH) concentrations of 0.1 M, 0.5 M, and 4 M, was optimized. A ratio of 1:2 (w/v) (weight of BSG by volume of alkali solution) at room temperature for 24 h was preferred to minimize reagents and energy consumption. To fully integrate the process, alkaline extracts were acidified to pH3 with citric acid, to obtain the protein-rich fractions. The AX were recovered by ethanol precipitation and citric acid and ethanol were recycled. This integrated extraction process allowed a yield of 82-85% of the BSG total proteins and 66-73% of total AX with formation of a cellulose rich residue almost devoid of nitrogen.