Development and application of a new Silent reporter system to quantitate the activity of enhancer elements in the type II Collagen Gene
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- 作者：Kazuo Itoa ; b ; Tamayuki Shinomuraa ; t.shinomura.trg@tmd.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：βgyg ; a β-galactosidase-hygromycin phosphotransferase fusion gene ; RCS ; an established cell line from the Swarm rat chondrosarcoma ; X-gal ; 5-bromo-4-chloro-3-indolyl-β-D-galactoside ; Col2a1 ; a gene encoding type II collagen alpha 1 chain ; PCR ; polymerase chain reaction
- 刊名：Gene
- 出版年：2016
- 出版时间：1 July 2016
- 年：2016
- 卷：585
- 期：1
- 页码：13-21
- 全文大小：1385 K
文摘
- istitem" id="list_l0005">
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id="p0005">We developed a novel reporter assay system to quantify enhancer activities.
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id="p0010">The system is based on a silent reporter gene integrated in a chromosome.
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id="p0015">Activities of different enhancer elements were systematically compared.
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id="p0020">Col2a1 enhancers E1 and E2 exhibit additive and synergistic effect, respectively.