Visualized detection of single-base difference in multiplexed loop-mediated isothermal amplification amplicons by invasive reaction coupled with oligonucleotide probe-modified gold nanoparticles

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• 作者：Yan Lu^a ; ¹ ; Xueping Ma^a ; ¹ ; Jianping Wang^a ; Nan Sheng^b ; Tianhui Dong^b ; Qinxin Song^c ; Jianzhong Rui^a ; Bingjie Zou^a ; ^{zbj523@163.com} ; Guohua Zhou^a ; ^{ghzhou@nju.edu.cn}
• 关键词：mLAMP ; Invasive reaction ; AuNPs ; Visualized detection
• 刊名：Biosensors and Bioelectronics
• 出版年：2017
• 出版时间：15 April 2017
• 年：2017
• 卷：90
• 期：Complete
• 页码：388-393
• 全文大小：486 K
• 卷排序：90

文摘

Visualized genotyping of multiple targets was achieved by detecting multiplexed LAMP amplicons with series target-specific invasive reactions coupled with hybridization-induced assembly of gold nanoparticles. The key steps of the method include sequence amplifier to amplify the multiple targets, allele identifier to recognize a targeted base in the multiplexed amplicons, and signal generator to yield red color from disconnected gold nanoparticles. Since sequence amplifier and allele identifier occurs at a constant temperature, the method only needs a simple temperature controller, much suitable for source-limited regions to assist with gene-guided personalized medicine.