Use of protein G microcolumns in chromatographic immunoassays: A comparison of competitive binding formats
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- 作者：Erika L. Pfaunmiller ; Jeanethe A. Anguizola ; Mitchell L. Milanuk ; NaTasha Carter ; David S. Hage ; dhage1@unl.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Chromatographic immunoassay ; Affinity microcolumn ; Protein G ; Competitive binding immunoassay ; Human serum albumin
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：1021
- 期：Complete
- 页码：91-100
- 全文大小：1431 K
文摘
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Protein G microcolumns were developed for use in chromatographic immunoassays.
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Human serum albumin was used as a model protein target for this work.
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Several solution- and column-based competitive binding formats were compared.
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These methods were compared in terms of their response, speed, and precision.
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The guidelines that were created can be used to extend this approach to other targets.