Sensitizing Pseudomonas aeruginosa to antibiotics by electrochemical disruption of membrane functions

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• 作者：Tagbo H.R. Niepa^a ; ^b ; Laura M. Snepenger^a ; ^b ; Hao Wang^a ; ^b ; Shiril Sivan^a ; ^b ; Jeremy L. Gilbert^a ; ^b ; Marcus B. Jones^c ; ^d ; Dacheng Ren^a ; ^b ; ^e ; ^f ; ^{dren@syr.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Persister cells ; Membrane ; Gene expression ; TEM ; SEM-EDS ; Electrodes
• 刊名：Biomaterials
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：74
• 期：Complete
• 页码：267-279
• 全文大小：1592 K

文摘

Recently, we reported synergistic effects between 70 渭A/cm² direct current and tobramycin in killing Pseudomonas aeruginosa PAO1 persister cells, a phenomenon we named electrochemical control of persister cells (ECCP; Niepa et al. Biomaterials 33: 7356–7365, 2012). To understand the mechanism of ECCP, the effects of electrochemical treatments mediated via stainless steel 304 and carbon electrodes on P. aeruginosa PAO1 were systematically compared using complementary approaches in this study. Electron microscopic analysis revealed that 渭A/cm² level direct current (DC) caused substantial changes in the structure and membrane integrity of P. aeruginosa PAO1 cells. DC treatments using SS304 electrodes induced cell lysis, while the same level of DC generated using carbon electrodes led to aggregation of intracellular proteins and increased permeabilization of P. aeruginosa PAO1 cells to antibiotics. The profound effects of DC on the physiology of persister cells were corroborated with DNA microarray analysis, which revealed the induction of genes associated with pyocin production and SOS response in DC-treated persister cells. Interestingly, sequential treatment using DC mediated with carbon electrodes followed by tobramycin was found more effective than concurrent treatment; and total eradication of persister cells was achieved.