A functional genomics tool for the Pacific bluefin tuna: Development of a 44K oligonucleotide microarray from whole-genome sequencing data for global transcriptome analysis
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- 作者：Motoshige Yasuike ; yasuike@affrc.go.jp" class="auth_mail" title="E-mail the corresponding author ; Atushi Fujiwara ; Yoji Nakamura ; Yuki Iwasaki ; Issei Nishiki ; Takuma Sugaya ; Akio Shimizu ; Motohiko Sano1 ; Takanori Kobayashi ; Mitsuru Ototake
- 关键词：PBT ; Pacific bluefin tuna ; ABT ; Atlantic bluefin tuna ; SBT ; southern bluefin tuna ; WGS ; whole-genome shotgun ; ESTs ; expressed sequence tags ; PBLs ; peripheral blood leukocytes ; LPS ; lipopolysaccharide ; poly I ; C ; polyinosinic ; polycytidylic acid ; NCBI ; National Center for Biotechnology Information ; nr ; non-redundant ; RefSeq ; Reference Sequence ; cDNA ; complementary DNA ; BLAST ; Basic Local Alignment Search Tool ; nt ; nucleotide ; UniProt ; Universal Protein Resource ; UniProtKB ; UniProt Knowledgebase ; GO
- 刊名：Gene
- 出版年：2016
- 出版时间：1 February 2016
- 年：2016
- 卷：576
- 期：2
- 页码：603-609
- 全文大小：666 K
文摘
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We developed the first 44K Pacific bluefin tuna (PBT) Thunnus orientalis oligonucleotide microarray (oligo-array).
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The PBT oligo-array successfully revealed distinct immune processes between LPS - and poly I:C- stimulated PBLs.
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We expect that this oligo-array is a valuable new tool for large-scale transcriptome studies in the PBT research community.