An electrochemical DNA biosensor based on Oracet Blue as a label for detection of Helicobacter pylori

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• 作者：Saeedeh Hajihosseini^a ; Navid Nasirizadeh^b ; ^{nasirizadeh@iauyazd.ac.ir" class="auth_mail" title="E-mail the corresponding author} ; Mohammad Saeid Hejazi^c ; Parichereh Yaghmaei^a
• 关键词：DNA biosensor ; Oracet Blue
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：91
• 期：Complete
• 页码：911-917
• 全文大小：1495 K

文摘

An innovative method of a DNA electrochemical biosensor based on Oracet Blue (OB) as an electroactive label and gold electrode (AuE) for detection of Helicobacter pylori, was offered. A single–stranded DNA probe with a thiol modification was covalently immobilized on the surface of the AuE by forming an Au–S bond. Differential pulse voltammetry (DPV) was used to monitor DNA hybridization by measuring the electrochemical signals of reduction of the OB binding to double–stranded DNA (ds–DNA). Our results showed that OB–based DNA biosensor has a decent potential for detection of single–base mismatch in target DNA. Selectivity of the proposed DNA biosensor was further confirmed in the presence of non–complementary and complementary DNA strands. Under optimum conditions, the electrochemical signal had a linear relationship with the concentration of the target DNA ranging from 0.3 nmol L⁻¹ to 240.0 nmol L⁻¹, and the detection limit was 0.17 nmol L⁻¹, whit a promising reproducibility and repeatability.