Genetic encoding of fluorescent RNA ensures a bright future for visualizing nucleic acid dynamics

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• 作者：Jason Dictenberg ^{dictenberg@genectr.hunter.cuny.edu}
• 刊名：Trends in Biotechnology
• 出版年：2012
• 出版时间：December, 2012
• 年：2012
• 卷：30
• 期：12
• 页码：621-626
• 全文大小：625 K

文摘

Recently RNA localization has been appreciated as an essential post-transcriptional mechanism to program local proteome composition and function. Although RNA has been visualized using diverse techniques, the use of the bacteriophage MS2 method to encode genetically fluorescent RNA has revolutionized the study of RNA dynamics in living cells. Here, I highlight the strength of MS2 compared to other techniques, and how further evolution of this system will enable the visualization of RNA in the context of complex live-cell dynamics. Although the generation of MS2-fluorescence resonance energy transfer (FRET) and MS2-bifluorescence complementation (BiFC) will require further development, it has the potential to increase significantly the signal-to-noise ratio, which is the major obstacle to rapid live-cell imaging of RNA.