Meningococcal serogroup Y lpxL1 variants from South Africa are associated with clonal complex 23 among young adults

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• 作者：Mignon du Plessis^a ; ^b ; ^c ; ^{mignond@nicd.ac.za" class="auth_mail} ; Nicole Wolter^a ; ^b ; ^c ; Penny Crowther-Gibson^a ; Hendrik-Jan Hamstra^d ; Kim Schipper^e ; Chivonne Moodley^a ; ^b ; Cheryl Cohen^a ; ^f ; Diederik van de Beek^g ; Peter van der Ley^d ; Anne von Gottberg^a ; ^b ; ^c ; Arie van der Ende^e ; ^h
• 关键词：Neisseria meningitidis ; South Africa ; lpxL ; Serogroup Y ; Clonal complex 23 ; MLST
• 刊名：Journal of Infection
• 出版年：May, 2014
• 年：2014
• 卷：68
• 期：5
• 页码：455-461
• 全文大小：909 K

文摘

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Summary

Objectives

To determine the genotypes of serogroup Y meningococcus (MenY), and to determine the prevalence of and identify factors associated with MenY lpxL1 variants.

Methods

Isolates, collected from 2003 to 2007 through national surveillance for invasive meningococcal disease, were characterized by multilocus sequence typing and screened for interleukin-6 induction. LpxL1 genes were sequenced from low IL-6 inducers.

Results

MenY represented 13% (n聽=聽219/1702) of meningococcal disease. Clonal complex (cc) 175, ST-23/Cluster A3 (cc23), cc11 and cc167 accounted for 82% (176/214), 11% (24/214), 3% (6/214) and 3% (7/214) respectively. Low cytokine induction was evident in 15% (32/218). Cc23 isolates (24/24) had an lpxL1 mutation, while among the remaining isolates the proportion of lpxL1 variants was 4% (8/189, p聽<聽0.001), and these were all cc175. Compared to wild type isolates, lpxL1 variants were associated with patients aged 5-14 years [unadjusted OR (95% CI): 4.3 (1.5-12)] or 15-24 years [unadjusted OR (95% CI): 9.1 (2.8-29)] compared to children <5 years; and were more likely have been isolated from CSF than blood [unadjusted OR (95% CI): 3.5 (1-11.9)]. On multivariable analysis, age remained significant [adjusted OR (95% CI), 5-14 years: 4.2 (1.5-12); 15-24 years: 8.9 (2.7-29)].

Conclusion

LpxL1 variants were associated with cc23 among young adults.