Molecular and functional changes in glucokinase expression in the brainstem dorsal vagal complex in a murine model of type 1 diabetes
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- 作者：K.C. Halmosa ; b ; P. Gyarmatia ; H. Xua ; S. Maimaitia ; G. Jancsó ; b ; G. Benedekb ; B.N. Smitha ; bret.smith@uky.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ACSF ; artificial cerebrospinal fluid ; CT ; cycle threshold ; DMV ; dorsal motor nucleus of the vagus ; EDTA ; Ethylenediaminetetraacetic acid ; EGFP ; enhanced green fluorescent protein ; EPSC ; excitatory postsynaptic current ; GA ; glucosamine ; GCK ; glucokinase ; IPSC ; inhibitory postsynaptic current ; KATP ; ATP-sensitive K+ channels ; NTS ; nucleus tractus solitarius ; RT-PCR ; reverse transcriptase-polymerase chain reaction ; STZ ; streptozotocin ; TTX ; tetrodotoxin
- 刊名：Neuroscience
- 出版年：2015
- 出版时间：15 October 2015
- 年：2015
- 卷：306
- 期：Complete
- 页码：115-122
- 全文大小：572 K
文摘
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Expression of glucokinase (GCK) in the dorsal vagal complex was significantly reduced in diabetic mice.
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Molecular expression of KATP channel components was unchanged.
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Synaptic responses of NTS neurons to GCK inhibition were significantly altered.
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Effects of increased [glucose] on action potential firing was attenuated.
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Reduced GCK-dependent, glucose-mediated responses in the NTS likely contribute to autonomic dysregulation in diabetes.