Validated spectrophotometric methods for the determination of bifonazole in pharmaceuticals by charge transfer complexation

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• 作者：Nasrin Banu Shaikh Ismail ; Badiadka Narayana ; ^{nbadiadka@yahoo.co.uk" class="auth_mail" title="E-mail the corresponding author} ; Kumble Divya
• 关键词：Bifonazole ; Charge transfer complexation ; 2 ; 5-Dichloro-3 ; 6-dihydroxy-1 ; 4-benzoquinone ; 2 ; 3-Dichloro-5 ; 6-dicyano-1 ; 4-benzoquinone ; Spectrophotometry ; FT-IR characterization
• 刊名：Journal of the Association of Arab Universities for Basic and Applied Sciences
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：19
• 期：Complete
• 页码：8-14
• 全文大小：1124 K

文摘

Two simple and selective visible spectrophotometric methods were developed for assay of bifonazole in pure drug and in its pharmaceutical formulation. The developed methods were based on the charge transfer complexation reaction of bifonazole with 2,5-dichloro-3,6-dihydroxy-1,4-benzoquinone (CAA) for method A and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) for method B, resulting in the formation of colored complex. The colored reaction products were quantitated spectrophotometrically at 517 nm and 457 nm for bifonazole-CAA and bifonazole-DDQ complexes, respectively. The complexes obeyed Beer’s law in the concentration range of 50.00–400.00 and 5.00–50.00 μg mL⁻¹ with molar absorptivities at 0.0956 × 10⁴ and 0.6953 × 10⁴ L mol⁻¹ cm⁻¹ for method A and method B, respectively. The proposed procedures were successfully applied for the quantitative determination of bifonazole in its pharmaceutical formulation. The solid charge transfer complexes of bifonazole with each of the π-acceptor reagent were also synthesized and characterized by FT-IR spectroscopy.