Investigating effects of magnetic nanoparticles on adsorption properties of beads. Specific surface area of magnetic beads is significantly more than nonmagnetic ones. Cibacron Blue F3GA (CB) was used as affinity ligand. CB density on magnetic beads is more than nonmagnetic ones. Protein adsorption and separation properties were investigated. The static and dynamic adsorption capacity was influenced by magnetic nanoparticles.