Chondroitin sulfate B is the major matrix component of bovine peritubular dentin.
Laser capture of PTD and ITD sections allowed mapping of elemental content; Ca; S ;P.
PTD and ITD differed quantitatively in elemental composition: SPTD > SITD; CaPTD > CaITD.
The PTD-ITD boundary is marked by enhanced PTD topographical surface roughness.
We demonstrate the EDS mapping of mineralized tissue with sub-nanometer resolution.