Monomer composition of chitooligosaccharides obtained by different degradation methods and their effects on immunomodulatory activities

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• 作者：Ronge Xing^a ; ^b ; ^{xingronge@qdio.ac.cn} ; Yongliang Liu^a ; ^b ; ^c ; Kecheng Li^a ; ^b ; Huahua Yu^a ; ^b ; Song Liu^a ; ^b ; Yue Yang^a ; ^b ; Xiaolin Chen^a ; ^b ; Pengcheng Li^a ; ^b ; ^{pcli@qdio.ac.cn}
• 关键词：COS ; chitooligosaccharides ; GlcN ; glucosamine ; CTS ; chitosan ; YCOS ; chitooligosaccharide by degraded enzyme method ; ZCOS ; chitooligosaccharide by degraded traditional methods ; WCOS ; chitooligosaccharide by degraded microwave irradiation ; MTT ; 3-(4 ; 5-dimethylthiazol-z-yl)-2 ; 5-diphenyltetrazolium bromide ; ConA ; concanavalin A ; LPS ; lipopolysaccharide ; DMSO ; dimethyl sulphoxide ; FCS ; fetal calf serum ; HPLC ; high performance liquid phase chromatography ; GPC ; gel permeation chromatography ; PBS ; phosp
• 刊名：Carbohydrate Polymers
• 出版年：2017
• 出版时间：10 February 2017
• 年：2017
• 卷：157
• 期：Complete
• 页码：1288-1297
• 全文大小：1009 K
• 卷排序：157

文摘

Three kinds of chitooligosaccharides were prepared through different methods. Their monomer compositions were researched by GPC, MS and elemental analysis. Oligosaccharides by different methods could significantly stimulate the immune system. WCOS obtained by microwave irradiation had the best immunomodulatory activities. Activity of WCOS might relate to hexamer and acetyl group in molecule composition.