文摘
Mutant proteins in N-glycosylation sites, that were abolished in Aam1 or inserted into Cqm1, were generated. Aam1 has four N-glycosylated sites localized externally on its structure, while N-glycans seemed absent from Cqm1. Aam1 has higher catalytic efficiency than Cqm1, and carbohydrates removal from Aam1 changed the catalytic activity. N-glycosylation can enhance the functional diversity of these related orthologs proteins.