Rapid detection of cefotaxime-resistant Escherichia coli by LC-MS

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• 作者：A. Burrer^a ; P. Findeisen^a ; ^{peter.findeisen@medma.uni-heidelberg.de" class="auth_mail" title="E-mail the corresponding author} ; E. Jä ; ger^a ; B. Ghebremedhin^b ; A. Grundt^a ; P. Ahmad-Nejad^b ; T. Miethke^c ; M. Neumaier^a
• 关键词：AST ; antibiotic susceptibility testing ; AMP ; ampicillin ; AUC ; area under the curve ; CAZ ; ceftazidime ; CLSI ; Clinical and Laboratory Standards Institute ; CTX ; cefotaxime ; E. coli ; Escherichia coli ; HPLC ; high performance liquid chromatography ; LC ; liquid chromatography ; MAAST ; mass spectrometry-based antibiotics susceptibility testing ; MALDI-TOF ; matrix-assisted laser desorption ionization-time of flight ; MIC ; minimal inhibitory concentration ; MS ; mass spectrometry ; SPZ ; sulfaphenazole
• 刊名：International Journal of Medical Microbiology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：305
• 期：8
• 页码：860-864
• 全文大小：566 K

文摘

Antibiotic resistance is an unsolved healthcare problem with increasing impact on patient management in the last years. In particular, multidrug resistance among Gram-negative bacterial strains has become the most pressing challenge. In order to deliver the most efficacious antimicrobial therapy with minimum delay, rapid diagnostic tests are required in order to detect multidrug resistant pathogens early during infection. In line with these efforts, we have developed a mass spectrometry-based assay for the rapid determination of ampicillin and cefotaxime resistance. The assay quantifies beta-lactamase activities towards ampicillin and cefotaxime within a turnaround time of 150 min, which is substantially faster than classical susceptibility testing.