Correlation between the antibacterial activity and the composition of extracts derived from various Spanish Cistus species

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• 作者：Laura Tomá ; s-Menor^a ; Ará ; nzazu Morales-Soto^b ; ^c ; Enrique Barrajó ; n-Catalá ; n^a ; ^d ; Cristina Roldá ; n-Segura^c ; ^e ; Antonio Segura-Carretero^b ; ^c ; Vicente Micol^a ; ^{vmicol@umh.es}
• 关键词：FD ; freeze drying ; FRAP ; ferric-reducing ability power ; GAE ; gallic acid equivalents ; MBC ; minimum bactericidal capacity ; MIC50 ; minimum inhibitory concentration required to inhibit 50 % of bacterial growth ; ORAC ; oxygen radical absorbance capacity ; RRLC&n
• 刊名：Food and Chemical Toxicology
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：55
• 期：Complete
• 页码：313-322
• 全文大小：1915 K

文摘

Cistaceae is a large family of shrubs commonly distributed in the Mediterranean ecosystem. The aim of this study was to explore the potential antimicrobial properties against Escherichia coli and/or Staphylococcus aureus of different extracts obtained from four Cistaceae species that are especially abundant in Spanish semi-arid regions. MIC₅₀ values of the extracts of C. salviifolius exhibited potent bacteriostatic effects against S. aureus compared with the other Cistus species tested. Spray-drying had less impact on the antimicrobial activities and polyphenolic contents than did evaporation followed by freeze-drying. When C. salviifolius extract was concentrated and the polar fraction was removed, its bacteriostatic and bactericidal activities against both strains were significantly enhanced. Seasonal influences on the composition have also been found. Up to 48 compounds were found in the aqueous extract of C. salviifolius using RRLC-ESI-TOF-MS. The analysis of the composition of the extracts revealed that the inhibitory activity against E. coli may be related to the presence of galloylated flavanols and specific flavonols, whereas the inhibitory capacity against S. aureus may be related primarily to polar compounds and to other flavonols. Potential synergistic effects among polyphenols may deserve further studies. These extracts may serve as an alternative source of antimicrobial ingredients focused on medical devices or cosmetics.