High content analysis assay for prediction of human hepatotoxicity in HepaRG and HepG2 cells
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- 作者：Junichiro Saitoa ; junichiroh.saitoh@astellas.com" class="auth_mail" title="E-mail the corresponding author ; Ai Okamuraa ; Kenichiro Takeuchia ; Kenichi Haniokaa ; Akinobu Okadaa ; Takeji Ohatab
- 关键词：DILI ; drug-induced liver injury ; GLP ; good laboratory practice ; HCA ; high content analysis ; ROS ; reactive oxygen species ; GSH ; glutathione ; MMP ; mitochondrial membrane potential ; MP ; membrane permeability ; CM-H2DCFDA ; 5-(and-6)-chloromethyl-2&prime ; 7&prime ; -dichlorodihydrofluorescein diacetate ; acetyl ester ; TMRM ; tetramethylrhodamine methyl ester ; mBCl ; monochlorobimane ; SD ; standard deviation ; CV ; coefficient of variation ; LOAEC ; lowest observable adverse effect concentration ; CVB ; covalent b
- 刊名：Toxicology in Vitro
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：33
- 期：Complete
- 页码：63-70
- 全文大小：711 K
文摘
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HCA assay successfully detected clinical DILI potential in HepaRG and HepG2 cells.
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HepaRG and HepG2 cells had different responses to clinical DILI compounds.
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The known biological changes related to clinical DILI were detected in HCA assay.
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HepaRG and HepG2 cells should be used in HCA assays for hepatotoxic evaluation.