Single channel activity of OmpF-like porin from Yersinia pseudotuberculosis
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- 作者：Tatyana I. Rokitskayaa ; Elena A. Kotovaa ; Gennadiy A. Naberezhnykhb ; Valentina A. Khomenkob ; Vladimir I. Gorbachb ; Alexander M. Firsova ; c ; Elena A. Zelepugab ; Yuri N. Antonenkoa ; antonen@genebee.msu.ru" class="auth_mail" title="E-mail the corresponding author ; Olga D. Novikovab ; novolga_05@mail.ru" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bacterial porin ; Outer membrane protein ; Planar bilayer lipid membrane ; Ionic current ; Ion channel ; pH dependence ; Lysophosphatidylcholine ; Liposome
- 刊名：Biochimica et Biophysica Acta (BBA)-Biomembranes
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：1858
- 期：4
- 页码：883-891
- 全文大小：1124 K
文摘
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Channel activity of Y. pseudotuberculosis OmpF-like porin (YOmpF) was pH-sensitive.
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YOmpF channel-forming potency drastically decreased upon acidification.
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Acidification reduced single channel conductance of YOmpF reconstituted at neutral pH.
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YOmpF exhibited open-channel subconductance states at acidic pH.
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Addition of lysophosphatidylcholine also induced the subconductance states of YOmpF.