Conformational Selection in a Protein-Protein Interaction Revealed by Dynamic Pathway Analysis
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- 作者：Kalyan S. Chakrabarti1 ; 2 ; 5 ; 6 ; Roman V. Agafonov1 ; 2 ; 5 ; Francesco Pontiggia1 ; 2 ; Renee Otten1 ; 2 ; Matthew K. Higgins3 ; Gebhard F.X. Schertler4 ; Daniel D. Oprian2 ; oprian@brandeis.edu" class="auth_mail" title="E-mail the corresponding author ; Dorothee Kern1 ; 2 ; dkern@brandeis.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：conformational ensemble ; conformational selection ; energy landscape ; molecular recognition dynamics ; protein/protein interaction ; recoverin
- 刊名：Cell Reports
- 出版年：2016
- 出版时间：5 January 2016
- 年：2016
- 卷：14
- 期：1
- 页码：32-42
- 全文大小：2610 K
文摘
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Recoverin binds rhodopsin kinase by conformational selection
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NMR and stopped flow kinetics reveal binding mechanism
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Direct experimental distinction between conformational selection versus induced fit
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Binding-competent state with exposed hydrophobic binding pocket is only 3% populated