Dynamic relationships between ribosomal conformational and RNA positional changes during ribosomal translocation

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• 作者：Ping Xie ; ^{pxie@aphy.iphy.ac.cn}
• 关键词：Biophysics ; Mathematical Bioscience
• 刊名：Heliyon
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：2
• 期：12
• 全文大小：1430 K
• 卷排序：2

文摘

Ribosomal translocation catalyzed by EF-G hydrolyzing GTP entails multiple conformational changes of ribosome and positional changes of tRNAs and mRNA in the ribosome. However, the detailed dynamic relations among these changes and EF-G sampling are not clear. Here, based on our proposed pathway of ribosomal translocation, we study theoretically the dynamic relations among these changes exhibited in the single molecule data and those exhibited in the ensemble kinetic data. It is shown that the timing of these changes in the single molecule data and that in the ensemble kinetic data show very different. The theoretical results are in agreement with both the available ensemble kinetic experimental data and the single molecule experimental data.