Gene transcription profiles, global DNA methylation and potential transgenerational epigenetic effects related to Zn exposure history in Daphnia magna

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• 作者：Michiel B. V ; egehuchte ; Dieter De Coninck ; Tine V ; enbrouck ; Wim M. De Coen ; Colin R. Janssen
• 关键词：Ecotoxicology ; Hypomethylation ; Microarray ; Multigeneration ; Zinc
• 刊名：Environmental Pollution
• 出版年：2010
• 出版时间：October 2010
• 年：2010
• 卷：158
• 期：10
• 页码：3323-3329
• 全文大小：856 K

文摘

A reduced level of DNA methylation has recently been described in both Zn-exposed and non-exposed offspring of Daphnia magna exposed to Zn. The hypothesis examined in this study is that DNA hypomethylation has an effect on gene transcription. A second hypothesis is that accumulative epigenetic effects can affect gene transcription in non-exposed offspring from parents with an exposure history of more than one generation. Transcriptional gene regulation was studied with a cDNA microarray. In the exposed and non-exposed hypomethylated daphnids, a large proportion of common genes were similarly up- or down-regulated, indicating a possible effect of the DNA hypomethylation. Two of these genes can be mechanistically involved in DNA methylation reduction. The similar transcriptional regulation of two and three genes in the F₀ and F₁ exposed daphnids on one hand and their non-exposed offspring on the other hand, could be the result of a one-generation temporary transgenerational epigenetic effect, which was not accumulative.