Rat brain sagittal organotypic slice cultures as an ex vivo dopamine cell loss system

详细信息    查看全文

• 作者：Amy McCaughey-Chapman ; Bronwen Connor ; ^{b.connor@auckland.ac.nz}
• 关键词：Slice culture ; Dopamine ; Model ; Parkinson&rsquo ; s disease
• 刊名：Journal of Neuroscience Methods
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：277
• 期：Complete
• 页码：83-87
• 全文大小：925 K
• 卷排序：277

文摘

Rat brain organotypic slice cultures remain viable for up to 6 weeks in culture. Slice 3-dimensional cytoarchitecture is maintained over a 4 week culturing period. 6-hydroxydopamine induces a sustained loss of tyrosine hydroxylase expression. 6-hydroxydopamine progressively destroys Fluoro-Gold-positive nigral cells.