Development and validation of cell-based luciferase reporter gene assays for measuring neutralizing anti-drug antibodies against interferon beta
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- 作者：Christina Hermanruda ; Malin Rynera ; Thomas Luftb ; Poul Erik Jensenc ; Kathleen Ingenhovend ; Dorothea Rate ; Florian Deisenhammerb ; Per Soelberg Sø ; rensenc ; Marc Pallardyf ; Dan Sikkemag ; Elisa Bertottih ; Daniel Kramere ; Paul Creekei ; Anna Fogdell-Hahna ; Anna.Fogdell-Hahn@ki.se" class="auth_mail" title="E-mail the corresponding author ; on behalf of the ABIRISK consortium
- 关键词：Anti-drug antibodies ; Neutralizing antibodies ; Bioassay ; Luciferase ; Interferon beta ; Multiple sclerosis
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：430
- 期：Complete
- 页码：1-9
- 全文大小：686 K
文摘
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We redeveloped and validated two anti-human IFNβ luciferase reporter-gene assays.
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The optimal serum concentration in regards to sensitivity and recovery was 2.5%.
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A floating cut-point was implemented to identify IFNβ specific NAbs.
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The assays demonstrated high specificity and acceptable sensitivity.