Molecular characterization and expression profiles of MaCOL1, a CONSTANS-like gene in banana fruit

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• 作者：Jiao Chen^a ; ¹ ; Jian-ye Chen^a ; ¹ ; Jun-ning Wang^b ; Jian-fei Kuang^a ; Wei Shan^a ; Wang-jin Lu^a ; ^{wjlu@scau.edu.cn}
• 关键词：CO/COL ; CONSTANS/CONSTANS-like ; CaMV ; cauliflower mosaic virus ; FT ; FLOWERING LOCUS T ; GFP ; green fluorescent protein ; 1-MCP ; 1-Methylcyclopropene ; ORF ; open reading frame ; PEG ; polyethylene glycol ; qPCR ; quantitative real-time PCR ; RH ; relative humidity
• 刊名：Gene
• 出版年：2012
• 出版时间：1 April 2012
• 年：2012
• 卷：496
• 期：2
• 页码：110-117
• 全文大小：1063 K

文摘

CONSTANS (CO) gene is a key transcription regulator that controls the long-day induction of flowering in Arabidopsis plant. However, CO gene involved in fruit ripening and stress responses is poorly understood. In the present study, a novel cDNA encoding CONSTANS-like gene, designated as MaCOL1 was isolated and characterized from banana fruit. The full length cDNA sequence was 1887 bp with an open reading frame (ORF) of 1242 bp, encoding 414 amino acids with a molecular weight of 46.20 kDa and a theoretical isoelectric point of 5.40. Sequence alignment showed that MaCOL1 contained two B-box zinc finger motifs and a CCT domain. In addition, MaCOL1 showed transcriptional activity in yeast and was a nucleus-localized protein. Real-time PCR analysis showed that MaCOL1 was differentially expressed among various banana plant organs, with higher expression in flower. Expression of MaCOL1 in peel changed slightly, while accumulation of MaCOL1 transcripts in pulp obviously increased during natural or ethylene-induced fruit ripening, suggesting that MaCOL1 might be associated with the pulp ripening of banana fruit. Moreover, accumulation of MaCOL1 transcript was obviously enhanced by abiotic and biotic stresses, such as chilling and pathogen Colletotrichum musae infection. Taken together, our results suggest that MaCOL1 is a transcription activator and may be involved in fruit ripening and stress responses.