Comparison of manual and automated cultures of bone marrow stromal cells for bone tissue engineering

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• 作者：Hirokazu Akiyama¹ ; Asako Kobayashi² ; Masaki Ichimura¹ ; Hiroshi Tone³ ; Masaru Nakatani¹ ; Minoru Inoue² ; Arinobu Tojo² ; Hideaki Kagami² ; ⁴ ; ^{kagami@ims.u-tokyo.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Automated cell culture system ; Bone marrow stromal cells ; Osteogenic potential ; Bone tissue engineering ; Regenerative medicine
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：120
• 期：5
• 页码：570-576
• 全文大小：1354 K

文摘

The development of an automated cell culture system would allow stable and economical cell processing for wider clinical applications in the field of regenerative medicine. However, it is crucial to determine whether the cells obtained by automated culture are comparable to those generated by manual culture. In the present study, we focused on the primary culture process of bone marrow stromal cells (BMSCs) for bone tissue engineering and investigated the feasibility of its automation using a commercially available automated cell culture system in a clinical setting. A comparison of the harvested BMSCs from manual and automated cultures using clinically acceptable protocols showed no differences in cell yields, viabilities, surface marker expression profiles, and in vivo osteogenic abilities. Cells cultured with this system also did not show malignant transformation and the automated process was revealed to be safe in terms of microbial contamination. Taken together, the automated procedure described in this report provides an approach to clinical bone tissue engineering.