Regulation of production of the blue pigment indigoidine by the pseudo γ-butyrolactone receptor FarR2 in Streptomyces lavendulae FRI-5

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• 作者：Yohanes Novi Kurniawan¹ ; Shigeru Kitani¹ ; Aya Iida¹ ; Asa Maeda¹ ; Jelger Lycklama a Nijeholt¹ ; Yong Jik Lee¹ ; Takuya Nihira¹ ; ² ; ^{nihira@icb.osaka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Streptomyces ; γ-Butyrolactone autoregulator ; Autoregulator receptor ; Pseudoreceptor regulator ; Indigoidine
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：121
• 期：4
• 页码：372-379
• 全文大小：1539 K

文摘

The γ-butyrolactone autoregulator signaling cascade is widely distributed among Streptomyces species as an important regulatory system of secondary metabolism. In Streptomyces lavendulae FRI-5, a γ-butyrolactone autoregulator IM-2 and the IM-2 specific receptor FarA control production of the blue pigment indigoidine together with two types of antibiotics: d-cycloserine and the nucleoside antibiotics. Here, we demonstrated by in silico analysis that farR2 (a farA homologue), which is located in a cluster of regulatory genes including farA, belongs to the family of pseudoreceptor regulator genes, and that the expression of farR2 is controlled by the IM-2/FarA regulatory system. Disruption of farR2 resulted in delayed production of indigoidine and in transcriptional derepression of the clustered far regulatory genes. Moreover, FarR2 bound to the FarA-binding sequences in the promoter regions of the regulatory genes that were downregulated by FarR2.