Evaluation of sensitivity of TaqMan RT-PCR for rubella virus detection in clinical specimens
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- 作者：Kiyoko Okamotoa ; Yoshio Moria ; yoshiom@nih.go.jp" class="auth_mail" title="E-mail the corresponding author ; Rika Komagomeb ; Hideki Naganob ; Masahiro Miyoshib ; Motohiko Okanob ; Yoko Aokic ; Atsushi Ogurad ; Chiemi Hottad ; Tomoko Ogawad ; Miwako Saikusae ; Hiroe Kodamaf ; Yoshihiro Yasuig ; Hiroko Minagawag ; Takako Kuratah ; Daiki Kanbayashih ; Tetsuo Kaseh ; Sachiko Muratai ; Komei Shirabei ; Mitsuhiro Hamasakij ; Takashi Katok ; Noriyuki Otsukia ; Masafumi Sakataa ; Katsuhiro Komasea ; Makoto Takedaa
- 关键词：Rubella ; TaqMan real-time RT-PCR ; Laboratory diagnosis
- 刊名：Journal of Clinical Virology
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：80
- 期：Complete
- 页码：98-101
- 全文大小：267 K
文摘
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We evaluated a TaqMan RT-PCR assay for the rubella surveillance system in Japan.
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We tested its sensitivity with all rubella virus genotypes and clinical specimens.
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Detection limits for the rubella virus genotypes ranged from 1 to 10 pfu/reaction.
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The sensitivity of the TaqMan RT-PCR was lower than the conventional nested RT-PCR.
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The assay is appropriate for throat swab and urine samples until 5 days of illness.