Magnesium oxide grafted carbon nanotubes based impedimetric genosensor for biomedical application

详细信息    查看全文

• 作者：Manoj Kumar Patel ; Md. Azahar Ali ; Saurabh Srivastava ; Ved Varun Agrawal ; S.G. Ansari ; Bansi D. Malhotra
• 关键词：DNA functionalization ; Genosensor ; Impedance spectroscopy ; Magnesium oxide ; Multi-walled carbon nanotubes ; Vibrio cholerae
• 刊名：Biosensors and Bioelectronics
• 出版年：2013
• 出版时间：15 December, 2013
• 年：2013
• 卷：50
• 期：Complete
• 页码：406-413
• 全文大小：2957 K

文摘

Nanostructured magnesium oxide (size<10 nm) grafted carboxyl (COOH) functionalized multi-walled carbon nanotubes (nMgO-cMWCNTs) deposited electrophoretically onto indium tin oxide (ITO) coated glass electrode and have been utilized for Vibrio cholerae detection. Aminated 23 bases single stranded DNA (NH₂-ssDNA) probe sequence (O1 gene) of V. cholerae has been covalently functionalized onto nMgO-cMWCNTs/ITO electrode surface using EDC-NHS chemistry. This DNA functionalized MgO grafted cMWCNTs electrode has been characterized using X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and electrochemical techniques. The results of XPS studies reveal that sufficient O-C?O groups present at the nMgO-cMWCNTs surface are utilized for DNA binding. The results of hybridization studies conducted with fragmented target DNA (ftDNA) of V. cholerae using electrochemical impedance spectroscopy (EIS) reveal sensitivity as 3.87 ¦¸ ng^?1 cm^?2, detection limit of ¡«21.70 ng ¦ÌL^?1 in the linear range of 100-500 ng ¦ÌL^?1 and stability of about 120 days. The proposed DNA functionalized nMgO-cMWCNTs nanomatrix provides a novel impedimetric platform for the fabrication of a compact genosensor device for biomedical application.