Regenerative fluorescence “turn-on” probe for biothiols through Cu(II)/Cu(I) redox conversion
详细信息 查看全文
- 作者：Kyung-Sik LeeaAuthor Vitae ; Jongmin ParkaAuthor Vitae ; Hee-Jun ParkaAuthor Vitae ; Young Keun ChungaAuthor Vitae ; Seung Bum ParkaAuthor Vitae ; Hae-Jo Kimb ; haejkim@hufs.ac.kr" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Ik-Soo Shinc ; extant@ssu.ac.kr" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Jong-In Honga ; jihong@snu.ac.kr" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Fluorescent probe ; Biothiols ; Copper(II)-coumarin complex ; Redox reaction ; Glutathione (GSH)
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：237
- 期：Complete
- 页码：256-261
- 全文大小：1282 K
文摘
- •
We report a fluorescent “turn-on” molecular probe for biothiols where a non-fluorescent copper(II) coumarin complex turns to a strongly fluorescent Cu(I) complex by thiol-containing amino acids (Cys, Hcy, GSH).
- •
The strongly fluorescent Cu(I) complex with biothiols can be returned back to its original probe form by chemical or electrochemical oxidation, enabling it reused as the thiol probe for several turn-overs.
- •
The probe can be also successfully used in fluorescent imaging of the cellular GSH.