Revealing the functional structure of a new PLA2 K49 from Bothriopsis taeniata snake venom employing automatic “de novo” sequencing using CID/HCD/ETD MS/MS analyses
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- 作者：Victor Corasolla Carregaria ; b ; corasolla@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Jie Daib ; Thiago Verano-Bragab ; Thalita Rochac ; Luis Alberto Ponce-Sotoa ; Sergio Marangonia ; Peter Roepstorffb
- 关键词：Bothriopsis taeniata ; PLA2 ; Peaks ; Mass spectrometry ; LTQ Orbitrap Velos ; Myotoxic activity ; Snake venom
- 刊名：Journal of Proteomics
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：131
- 期：Complete
- 页码：131-139
- 全文大小：1765 K
文摘
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We isolated a new phospholipase A2 K49. The entire amino acid sequence by was analyzed by mass spectrometry approach.
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Myotoxic studies elucidate the relation between structure/function of the PLA2K49.
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We identified the main importance of the lysine with the membrane anchorage.
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Propound a disrupt membrane mechanism by PLA2K49 in the myotoxic effect.