Quantification of bisnaphthalimidopropyldiaminooctane (BNIPDaoct) was attained.
A validated fluorometric HPLC method for biological matrices was developed.
Matrix effects were observed for spleen, liver, kidney, heart, and lung extracts.
LOD and LOQ values were ≤4 and ≤9 nmol g−1 in mice organs.
Application to biodistribution profile assessment is feasible.