A diagnosis of Burkholderia pseudomallei
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- 作者：Manuela Soares Couto ; Rossana de Aguiar Cordeiro ; Marcos Fá ; bio Gadelha Rocha ; Thalles Barbosa Grangeiro ; Natanael Pinheiro Leitã ; o Junior ; Tereza de Jesus Pinheiro Gomes B ; eira ; José ; J&#
- 关键词：Melioidosis ; Bronchoalveolar lavage ; PCR ; Diagnosis
- 刊名：Diagnostic Microbiology and Infectious Disease
- 出版年：2009
- 出版时间：September 2009
- 年：2009
- 卷：65
- 期：1
- 页码：73-75
- 全文大小：163 K
文摘
Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B agent by the US Centers for Disease Control and Prevention. The present article reports a diagnosis of B. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. The results obtained show that direct detection of the 16-23s spacer sequence in bronchoalveolar lavage is a quick and specific test to diagnose melioidosis.