The resolving power of in vitro genotoxicity assays for cigarette smoke particulate matter

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• 作者：K. Scott^a ; ^{ken_scott@bat.com} ; J. Saul^b ; ^{jim.saul@covance.com} ; I. Crooks^a ; ^{ian_crooks@bat.com} ; O.M. Camacho^a ; ^{oscar_m_camacho@bat.com} ; D. Dillon^a ; ^{debbie_dillon@bat.com} ; C. Meredith^a ; ^{clive_meredith@bat.com}
• 关键词：ANCOVA ; analysis of covariance ; CORESTA ; Cooperation Centre for Scientific Research Relative to Tobacco ; DMSO ; dimethyl sulphoxide ; ICH ; International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use
• 刊名：Toxicology in Vitro
• 出版年：2013
• 出版时间：June, 2013
• 年：2013
• 卷：27
• 期：4
• 页码：1312-1319
• 全文大小：630 K

文摘

In vitro genotoxicity assays are often used to compare tobacco smoke particulate matter (PM) from different cigarettes. The quantitative aspect of the comparisons requires appropriate statistical methods and replication levels, to support the interpretation in terms of power and significance. This paper recommends a uniform statistical analysis for the Ames test, mouse lymphoma mammalian cell mutation assay (MLA) and the in vitro micronucleus test (IVMNT); involving a hierarchical decision process with respect to slope, fixed effect and single dose comparisons. With these methods, replication levels of 5 (Ames test TA98), 4 (Ames test TA100), 10 (Ames test TA1537), 6 (MLA) and 4 (IVMNT) resolved a 30 % difference in PM genotoxicity.