Evaluation of the cytotoxicity of cigarette smoke condensate by a cellular impedance biosensor

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• 作者：Huan Chen^a ; ^{hunny_ch@163.com} ; Lin Cui^b ; Xing-Yi Jiang^a ; Yong-Qiang Pang^a ; Gang-Ling Tang^a ; Hong-Wei Hou^a ; Jian-Hui Jiang^c ; Qing-Yuan Hu^a ; ^{qyhu@ztri.com.cn}
• 关键词：ANOVA ; Analysis of Variance ; CHO ; Chinese hamster ovary ; CI ; cell index ; CIB ; cellular impedance biosensor ; CORESTA ; cooperation center for scientific research relative to tobacco ; CSCs ; cigarette smoke condensates ; CV ; coefficient of variation ; DMSO ; dim
• 刊名：Food and Chemical Toxicology
• 出版年：2012
• 出版时间：March-April, 2012
• 年：2012
• 卷：50
• 期：3-4
• 页码：612-618
• 全文大小：761 K

文摘

In this study, a cytotoxicity assay was developed for profiling the cytotoxicity of cigarette smoke condensates (CSCs) base on a cellular impedance biosensor (CIB). Compared with the traditional in vitro cytotoxicity assays, this CIB-based method offered distinct advantages in real-time kinetic measurement which provided a comprehensive understanding of cellular responses for the entire duration of the experiment and prediction of the potential mechanism of action of a given treatment. The time-dependent cell response profiles provided valid evidences for optimization of cell number per well, cell quality control, and identification of the optimal time points for compound treatment and endpoint assays. According to the time dependent IC₅₀ values, the CIB could provide dynamic information that can be used to identify maximum toxicity of cigarette smoke and reversibility of the toxic effects which are difficult to achieve by the endpoint assays. The comparative IC₅₀ values indicated that the as-developed biosensor offered analytical results in good consistency with the commonly used NRU method. The features of the CIB-based cytotoxicity assay, such as no cell labeling, automatic detection, and easy operation, give this assay potential to become routine setting for evaluating the cytotoxicity of CSCs.