IL-10 and toll-like receptor-4 polymorphisms and the in vivo and ex vivo response to endotoxin

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• 作者：Schippers ; Emile F. ; van 't Veer ; Cornelis ; van Voorden ; Sjaak ; Martina ; Cerithsa A.E. ; Huizinga ; Tom W.J. ; et. al.
• 关键词：Cardiopulmonary bypass ; Endotoxemia ; Lipopolysaccharides ; Toll-like receptor-4 ; IL-10 promoter
• 刊名：Cytokine
• 出版年：2005
• 出版时间：March 7, 2005
• 年：2005
• 卷：29
• 期：5
• 页码：215-228
• 全文大小：239 K

文摘

To determine to what extent lipopolysaccharide-induced IL-10 production capacity is determined by polymorphisms in toll-like receptor-4 (TLR4) and the IL-10 promoter region, we measured in vivo IL-10 and TNF-α production in patients undergoing elective cardiopulmonary bypass surgery, a major surgical trauma associated with ischemia-reperfusion injury that triggers an endotoxemia and profound inflammatory response in most patients. Ex vivo the IL-10 and TNF-α production was measured in a whole blood stimulation assay, using 3 LPS concentrations. Positive correlations were found between TNF-α and IL-10 production ex vivo, upon stimulation with each of the LPS concentrations. Also, the estimated TNF-α and IL-10 EC₅₀, and TNF-α_max and IL-10_max were positively correlated (r