Phenotypic screen for RNAi effects in the codling moth Cydia pomonella
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- 作者：Jinda Wanga ; b ; Liuqi Gua ; Stephen Irelanda ; c ; Stephen F. Garczynskid ; Douglas C. Knipplea ; dck2@cornell.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：PCR ; polymerase chain reaction ; RT-PCR ; reverse transcriptase PCR ; qRT-PCR ; quantitative real-time PCR ; cDNA ; complementary DNA ; ANOVA ; analysis of variance ; RNAi ; RNA interference ; dsRNA ; double strand RNA ; SD ; standard deviation
- 刊名：Gene
- 出版年：2015
- 出版时间：10 November 2015
- 年：2015
- 卷：572
- 期：2
- 页码：184-190
- 全文大小：969 K
文摘
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We used RNAi to suppress expression of a cullin-1 homolog of the codling moth.
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Cpcul1-dsRNA caused dose-dependent suppression of Cpcul1 expression.
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Cpcul1-dsRNA caused stunting of larval growth but did not affect viability.