Preparation of a biocatalyst via physical adsorption of lipase from Thermomyces lanuginosus on hydrophobic support to catalyze biolubricant synthesis by esterification reaction in a solvent-free system
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- 作者：Flá ; via A.P. Lage ; Jaquelinne J. Bassi ; Maria C.C. Corradini ; Larissa M. Todero ; Jaine H.H. Luiz ; Adriano A. Mendes ; adriano_amendes@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author ; adriano.mendes@unifal-mg.edu.br" class="auth_mail" title="E-mail the corresponding author
- 关键词：Lipase immobilization ; Interfacial activation ; Poly-methacrylate particles ; Optimization ; Biolubricant synthesis ; Spectroscopy analyses
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：84
- 期：Complete
- 页码：56-67
- 全文大小：1463 K
文摘
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Lipase from Thermomyces lanuginosus was immobilized on poly-methacrylate particles.
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The biocatalysts were tested in isoamyl oleate (biolubricant) synthesis.
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A satisfactory combination of high catalytic activity and reusability was reached.
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Ester conversion ≈85% was reached after 30 min of reaction in a solvent-free system.
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The nature of the ester was characterized by spectroscopy analyses (ATR-FTIR and NMR).