Role of PACAP on testosterone and 17β-estradiol production in the testis of wall lizard Podarcis sicula

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• 作者：Luigi Rosati^a ; Marina Prisco^a ; Maria Maddalena Di Fiore^b ; Alessandra Santillo^b ; Salvatore Valiante^a ; Piero Andreuccetti^a ; ^{piero.andreuccetti@unina.it" class="auth_mail" title="E-mail the corresponding author} ; Marisa Agnese^a
• 关键词：PACAP ; Steroidogenesis ; Reptiles ; Sex steroid hormones ; PACAP receptor antagonists
• 刊名：Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：191
• 期：Complete
• 页码：180-186
• 全文大小：1395 K

文摘

Pituitary adenylate cyclase-activating peptide (PACAP) is a neuropeptide that in mammalian testis is involved in the control of testosterone and 17β-estradiol synthesis. A similar involvement was recently postulated in the testis of a nonmammalian vertebrate, the wall lizard Podarcis sicula. Indeed, we reported the presence of PACAP and its receptors throughout the reproductive cycle within both germ and somatic cells. Now, we investigated the effects of PACAP on steroidogenesis in significant periods of Podarcis reproductive cycle: winter stasis, reproductive period and summer stasis. Using different in vitro treatments, in the absence or presence of receptor antagonists, we demonstrated that in P. sicula testis PACAP is involved in the control of testosterone and 17β-estradiol production. In particular we demonstrated that treatment with PACAP induced a testosterone increase only in stasis periods (winter and summer stasis); differently they induced a 17β-estradiol production in all periods analyzed (summer stasis, winter stasis and reproductive period).